Estradiol, Progesterone and Cytokines Production
نویسنده
چکیده
OBJECTIVES: The purpose of this study was to determine the effects of estradiol and progesterone on interferon-γ (IFN-γ), interleukin (IL)-12, IL-10 and tumor necrosis factor-α (TNF-α) productions in polyclonal activators (phyto hemagglutinin+lipopolysaccharide)-stimulated whole blood cultures. METHODS : Nineteen healthy males and females volunteered in the study. Blood samples were drawn, diluted, and cultured for 24h with different concentrations of estradiol, progesterone or hydrocortisone and then PHA+LPS was added for another 24 h. The supernatant, then, was harvested and assayed for IL-12 p70, IFN-γ, IL-10 and TNF-α. RESULTS : At preovulatory concentrations, estradiol enhanced signifi cantly IFN-γ, IL-12 and IL-10, but not TNF-α, production levels and reversed the suppressive effect of hydrocortisone in PHA+LPS stimulated whole blood. While IL-10 levels kept increasing at pregnancy estradiol concentrations, IFN-γ, IL-12 levels and IFN-γ/IL-10 ratio decreased signifi cantly. No effect of progesterone on IL-12 p70, IFN-γ, IL-10 and TNF production levels was observed. CONCLUSIONS : The present study shows that those pregnancy estradiol concentrations (and higher) enhance the production of IL-10 and reduce IL-12, IFN-γ levels and IFN-γ/IL-10 ratio in stimulated whole blood cells. Because of the known IL-10 inhibitory actions on T helper (Th) 1 cells and monocytes/ macrophages, these high IL-10 levels keep Th2 cytokines favored during pregnancy and may be useful in shifting Th1-mediated autoimmune diseases towards non-pathogenic Th2 pathway. O
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تاریخ انتشار 2003